We collect and fix genital tissues from fly pupae at particular time points during development:
Download our detailed dissection protocol.
We design and synthesize antisense RNA probes that will bind messenger RNA for our target genes:
Download our detailed probe protocol.
We visualize gene expression patterns by applying probes to our samples and binding an antibody conjugated to an enzyme that catalyzes dye deposition:
We image our samples using light microscopy:
University of Pittsburgh
Vincent, B.J.*, Rice, G.R.*, Wong, G.M., Glassford, W.J., Downs, K.I., Shastay, J.L., Charles-Obi, K., Natarajan, M., Gogol, M.M., Zeitlinger, J., Rebeiz, M., 2019. An atlas of transcription factors expressed in the Drosophila melanogaster pupal terminalia. bioRxiv 677260. doi:10.1101/677260
*Since Gavin and Ben contributed equally to this work, please cite it as Rice and Vincent, et al. or Vincent and Rice, et al. We encourage you to flip a coin in deciding between those possibilities
This work is generously supported by NIH grant GM107387